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Q&A NEW CONTACT SUPPORT
  1. 10X Genomics
  2. General Single Cell RNA-seq
  3. Sample Prep - Cells

Sample Prep - Cells

  • What metrics should I be looking at to optimize my sample prep in my pilot experiment?
  • Should I use warm or cold tissue dissociation?
  • What are the best practices for flow sorting cells for 10x Genomics assays?
  • Are there considerations for small cells or cells with low RNA?
  • Are fresh frozen tissue samples compatible with Single Cell RNA sequencing?
  • Can viral transcripts be detected with the Single Cell Assays?
  • Can exosomes, synaptosomes and other extracellular vesicles be run in Single cell applications?
  • Are there any recommendations for working with post-mortem or biopsy tissue?
  • Does 10x have a demonstrated protocol for isolating PBMCs?
  • How do I prepare adherent cell lines for Single Cell analysis?
  • Can I process neutrophils (or other granulocytes) using 10x Single Cell applications?
  • Can transcriptional inhibitors (i.e. Actinomycin D) be used with the Single Cell Gene Expression workflow?
  • Can I use acetylated BSA in the cell washing and resuspension buffer?
  • How can I ship cells?
  • What cell strainers are recommended for single-cell assays?
  • Should I deplete red blood cells from my sample before loading?
  • How long can single cell or nuclei suspension be kept on ice?
  • Are cardiomyocytes compatible with Single-Cell assays?
  • Are RNase inhibitors required in the preparation of my sample?
  • Can cells be stained with dyes such as propidium iodide (PI) or Hoechst dyes?
  • Are Miltenyi Microbeads or EasySep beads compatible with the 10x Single Cell RNA-seq workflow?
  • Are there gene expression differences between fresh and frozen-thawed cells?
  • How do I prepare extremely precious or fragile cells?
  • What buffers can be used for washing and cell resuspension?
  • What is the minimum number of cells that can be profiled?
  • Can I add enzymes (such as DNase) or other chemical additives to my cell suspension?
  • What is the highest BSA concentration that can be used in the cell buffer?
  • How do I dissociate my tissue of interest?
  • How should I count my cells?
  • Can RNAlater or other RNA stabilization reagents be used?
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