Sample Prep - Cells
- Is there a recommended protocol for isolating PBMCs?
- How do I prepare adherent cell lines for Single Cell analysis?
- Can transcriptional inhibitors (i.e. Actinomycin D) be used with the Single Cell Gene Expression workflow?
- Can I use acetylated BSA in the cell washing and resuspension buffer?
- How can I ship cells?
- What cell strainers are recommended?
- Should I deplete red blood cells from my sample before loading?
- Can I sort my cells prior to running through the 10x Single Cell assay?
- How long can single cell or nucleus suspension be kept on ice?
- How can I test whether my cells will lyse in GEMs?
- Are cardiomyocytes compatible with Single Cell assays?
- Can I isolate nuclei from frozen tissue for gene expression profiling?
- Can RNase inhibitors be used in the preparation of my sample?
- Can cells be stained with dyes such as propidium iodide (PI) or Hoechst dyes?
- Are Miltenyi Microbeads or EasySep beads compatible with 10x Single Cell workflow?
- Are there gene expression differences between fresh and frozen-thawed cells?
- How do I prepare extremely precious or fragile cells?
- What buffers can be used for washing and cell resuspension?
- What is the minimum number of cells that can be profiled?
- Can I add enzymes (such as DNase) or other chemical additives to my cell suspension?
- What is the highest BSA concentration that can be used in the cell buffer?
- What is the range of compatible cell sizes?
- How do I dissociate my tissue of interest?
- How should I count my cells?
- Can RNAlater or other RNA stabilization reagents be used?
- How do I minimize background RNA during sample prep?
- Can cryopreserved cells be used for gene expression profiling?