Sample Prep - Cells
- What metrics should I be looking at to optimize my sample prep in my pilot experiment?
- Should I use warm or cold tissue dissociation?
- What are the best practices for flow sorting cells for 10x Genomics assays?
- Are there considerations for small cells or cells with low RNA?
- Are fresh frozen tissue samples compatible with Single Cell RNA sequencing?
- Can viral transcripts be detected with the Single Cell Assays?
- Can exosomes, synaptosomes and other extracellular vesicles be run in Single cell applications?
- Are there any recommendations for working with post-mortem tissue?
- Does 10x have a demonstrated protocol for isolating PBMCs?
- How do I prepare adherent cell lines for Single Cell analysis?
- Can I process neutrophils (or other granulocytes) using 10x Single Cell applications?
- Can transcriptional inhibitors (i.e. Actinomycin D) be used with the Single Cell Gene Expression workflow?
- Can I use acetylated BSA in the cell washing and resuspension buffer?
- How can I ship cells?
- What cell strainers are recommended for single-cell assays?
- Should I deplete red blood cells from my sample before loading?
- How long can single cell or nuclei suspension be kept on ice?
- Are cardiomyocytes compatible with Single-Cell assays?
- Are RNase inhibitors required in the preparation of my sample?
- Can cells be stained with dyes such as propidium iodide (PI) or Hoechst dyes?
- Are Miltenyi Microbeads or EasySep beads compatible with the 10x Single Cell RNA-seq workflow?
- Are there gene expression differences between fresh and frozen-thawed cells?
- How do I prepare extremely precious or fragile cells?
- What buffers can be used for washing and cell resuspension?
- What is the minimum number of cells that can be profiled?
- Can I add enzymes (such as DNase) or other chemical additives to my cell suspension?
- What is the highest BSA concentration that can be used in the cell buffer?
- What is the range of compatible cell sizes?
- How do I dissociate my tissue of interest?
- How should I count my cells?