GEM Generation & Barcoding
- Is it normal to observe settling of gel beads before loading the chip in Chromium assays?
- Is it normal to observe bright patches on the bottom surface of Chromium chips?
- Gel Bead Color Codes and Ordering for GEM-X Universal Multiplex (a.k.a On-Chip Multiplexing, OCM)
- What should I do if there is reagent on the Chromium X Series Chip Holder after a GEM-X run?
- How do I diagnose and report a wetting failure with GEM-X assays?
- How do I diagnose and report a clog failure with GEM-X assays?
- What if I accidentally fill the 'No fill' row with glycerol when loading a NextGEM chip?
- Can samples be treated with UV light, which is known to cross-link DNA? How would UV treatment affect RNA in the sample?
- Can an additional temperature incubation step be included during GEM-RT Incubation ?
- During GEM-RT Incubation in the Single Cell Assays, can the 85°C (step 2) be extended for more than 5 min?
- Can Trizol be used as a substitute for Recovery Agent?
- Will Recovery Agent (PN 220016) inactivate infectious agent/s in a sample when processing GEMs in the 10x workflow?
- Which thermal cyclers are supported for use with GEMs?