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  1. 10X Genomics
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  3. GEM Generation & Barcoding

GEM Generation & Barcoding

  • Is it normal to observe settling of gel beads before loading the chip in Chromium assays?
  • Is it normal to observe bright patches on the bottom surface of Chromium chips?
  • Gel Bead Color Codes and Ordering for GEM-X Universal Multiplex (a.k.a On-Chip Multiplexing, OCM)
  • What should I do if there is reagent on the Chromium X Series Chip Holder after a GEM-X run?
  • How do I diagnose and report a wetting failure with GEM-X assays?
  • How do I diagnose and report a clog failure with GEM-X assays?
  • What if I accidentally fill the 'No fill' row with glycerol when loading a NextGEM chip?
  • Can samples be treated with UV light, which is known to cross-link DNA? How would UV treatment affect RNA in the sample?
  • Can an additional temperature incubation step be included during GEM-RT Incubation ?
  • During GEM-RT Incubation in the Single Cell Assays, can the 85°C (step 2) be extended for more than 5 min?
  • Can Trizol be used as a substitute for Recovery Agent?
  • Will Recovery Agent (PN 220016) inactivate infectious agent/s in a sample when processing GEMs in the 10x workflow?
  • Which thermal cyclers are supported for use with GEMs?
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