Sequencing
- What is the limit of detection for a specific clonotype using the Single Cell Immune Profiling assay?
- Can I sequence my V(D)J libraries in a different sequencing configuration than the ones recommended in the user guide?
- When do I need to sequence my V(D)J-enriched libraries more deeply?
- Is there a common 5’ UTR captured during V(D)J enrichment?
- Should I adjust the targeted sequencing depth if I did not enrich for T or B cells?
- Can I sequence V(D)J-enriched libraries from T and B cells on the same lane?
- Can I sequence V(D)J and 5' Gene Expression libraries on the same lane?
- What Illumina sequencers are supported for Single Cell V(D)J libraries?