Sequencing
- Is it expected to see low abundance of Ts in cycles 1 and 10 of Read 1 when sequencing 5’ v3 pools?
- What sequencing configuration is needed for Chromium GEM-X Immune Profiling 5’ v3?
- What is the limit of detection for a specific clonotype using the Single Cell Immune Profiling assay?
- Can I sequence my V(D)J libraries in a different sequencing configuration than the ones recommended in the user guide?
- When do I need to sequence my V(D)J-enriched libraries more deeply?
- Is there a common 5’ UTR captured during V(D)J enrichment?
- Should I adjust the targeted sequencing depth if I did not enrich for T or B cells?