Post GEM-RT Cleanup & cDNA Amplification
- How can I optimize V(D)J primer design for my non-human/non-mouse species of interest?
- Why does my V(D)J enrichment trace look different from the user guide?
- Which genes does each V(D)J-specific primer map to?
- Why do I see big fragments (2,000- 9,000 bp) in my TCR-enriched trace?
- What is the difference between V(D)J Enrichment for Human and Mouse samples?
- Why is the distribution of fragments after amplification different between T and B cells?
- How do I design a custom PCR-based target enrichment assay for Single Cell V(D)J?
- Why are there two target enrichment steps in the V(D)J workflow?