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  3. Post GEM-RT Cleanup & cDNA Amplification

Post GEM-RT Cleanup & cDNA Amplification

  • How can I optimize V(D)J primer design for my non-human/non-mouse species of interest?
  • Which genes does each V(D)J-specific primer map to?
  • What is the difference between V(D)J Enrichment for Human and Mouse samples?
  • Why is the distribution of fragments after amplification different between T and B cells?
  • How do I design a custom PCR-based target enrichment assay for Single Cell V(D)J?
  • Why are there two target enrichment steps in the V(D)J workflow?
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