Questions & Answers
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Fixed RNA Profiling
- Can I pool less than 4 or 16 samples when using the Chromium Fixed RNA Profiling Reagent Kits for Multiplexed Samples?
- What is the maximum number of cells that can be targeted per barcode when using the Chromium Fixed RNA Profiling Reagent Kits for Multiplexed Samples?
- Can I ship fixed single cell/nuclei samples for use with the Fixed RNA Profiling Assay?
- Can I use fewer than the minimum recommended number of cells/nuclei during sample fixation or probe hybridization with the Fixed RNA Profiling Assay?
- Can the Enhancer be kept at 42°C or 65°C for longer periods of time?
- Is the Fixed RNA Profiling assay compatible with intracellular staining?
- Can formaldehyde from alternative vendors be used for sample fixation in the Fixed RNA Profiling Assay?
- Can I use formalin fixed tissue as input for the Gene Expression Flex assay?
- Can an FFPE tissue slide be used as input for the Isolation of Cells from FFPE Tissue Sections Demonstrated Protocol (CG000632)?
- Can I use Xylene alternatives for Tissue Deparaffinization during the Isolation of Cells from FFPE Tissue Sections Demonstrated Protocol (CG000632)?
- What are the validated stopping points for sample storage in the Fixed RNA Profiling assay?
- Why was the -20°C optional sample storage recommendation removed from the Fixed RNA Profiling assay?
GEM Generation & Barcoding
- Why does my Fixed RNA Profiling library look unexpected or have low yield?
- What is the expected size and concentration of Fixed RNA Profiling Libraries?
- Why do I need a different sample index plate (Dual Index Kit TT) to make Fixed RNA Profiling Feature Barcode libraries?
- What dual index plate is compatible with the Chromium Fixed RNA Profiling?
- Why was the percentage of PhiX for NovaSeq increased for Multiplexed Fixed RNA Profiling libraries sequenced on NovaSeq?
- Why are the "Reads Mapped Confidently to the Probe Set" or "Reads Mapped Confidently to the Filtered Probe Set" metrics in my Fixed RNA Profiling web summary file low?
- What sequencing parameters should be used for Fixed RNA Profiling gene expression libraries?
- Can I pool Fixed RNA Profiling gene expression libraries with other 10x Genomics libraries for sequencing?
- What Illumina sequencers are supported for Fixed RNA Profiling libraries?
- Is it expected to see a significant background signal from genomic DNA in Chromium Fixed RNA Profiling data?
- What does the term coverage mean in Fixed RNA Profiling?
- Is it possible to integrate fixed RNA profiling data with 3' or 5' gene expression data?
- Can I use PDX/xenograft tissue with Fixed RNA Profiling?
- Does Cell Ranger normalize UMI counts based on the coverage of genes in Fixed RNA Profiling?
- How do I use Cell Ranger aggr to aggregate Fixed RNA Profiling datasets?