Questions & Answers
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SupportTargeted
General
- Is the Immunofluorescence Staining Demonstrated Protocol for Visium for fresh-frozen compatible with the Targeted Gene Expression assay?
- Is the Targeted Gene Expression assay compatible with other species?
- Is the Targeted Gene Expression assay more sensitive than a whole transcriptome assay?
- Is the Targeted Gene Expression assay compatible with nuclei?
- Can non-coding RNAs be detected in the Targeted Gene Expression assay?
- Can I use the Targeted Gene Expression assay to detect specific polymorphisms?
Input Libraries
- Does the number of input libraries impact assay performance in the Targeted Gene Expression assay?
- How can I generate enough library mass to use as input into the Targeted Gene Expression assay?
- What library types can be pooled prior to hybridization in the Targeted Gene Expression assay?
- Which version of the Visium Spatial Gene Expression User Guide is supported for use with the Targeted Gene Expression assay?
- Why do I need to calculate cDNA length for the Spatial Targeted Gene Expression assay?
- Can I pool libraries generated from short cDNA and long cDNA for use in the Spatial Targeted Gene Expression assay?
Hybridization / Capture Workflow
- Can I use custom panels with the SPRIselect Library Concentration Protocol in the Targeted Gene Expression assay?
- Why are the Universal Blockers added after the SPRIselect step in Demonstrated Protocol CG000249?
- What are the sequences of the Library Amp Primers in the Target Hybridization Kit?
- What settings should be used for vacuum centrifugation in the Targeted Gene Expression assay?
- Are alternative streptavidin beads compatible with the Targeted Gene Expression assay?
- Can I use 1.5 ml tubes instead of 0.2 ml tubes for vacuum centrifugation in the Targeted Gene Expression assay?
Panel Selection
- How do I order custom panels for the Targeted Gene Expression assay?
- Can Twist Oligo Pools be used as add-on panels in the Targeted Gene Expression assay?
- How do I generate custom panels from Twist Oligo Pools for use in the Targeted Gene Expression assay?
- Are mouse pre-designed panels available for the Targeted Gene Expression assay?
- Are mouse panels supported in the Spatial Targeted Gene Expression assay?
- What are the bait design criteria for 10x pre-designed and custom panels?