Questions & Answers
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- What Demonstrated Protocols for sample preparation are compatible with 3' Feature Barcoding?
- Is there a recommended protocol for fixing PBMC samples?
- How can I test the quality of my nuclei prep?
- How does methanol fixation affect transcriptome data from cells?
- Is it possible to freeze nuclei prior to Single Cell 3'?
- Can gene expression in microbial samples be profiled?
- How can I convert the feature-barcode matrix from Cell Ranger 3.x to a CSV file?
- How can I convert the gene-barcode matrix from Cell Ranger 1.x and 2.x to a CSV file?
- How do I get the read counts for each barcode?
- How to view aggregated .cloupe file by library?
- How can the percentage of reads aligned to "Exonic Regions" be greater than those aligned to the "Transcriptome"?
- What to do if I sequenced more bases than required on the R1, R2, or the index read for 3' single-cell data?