Questions & Answers
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- How should I store my single-cell suspension for scRNA-seq?
- What Demonstrated Protocols for sample preparation are compatible with 3' Feature Barcoding?
- Is there a recommended protocol for fixing PBMC samples?
- How can I test the quality of my nuclei prep?
- How does methanol fixation affect transcriptome data from cells?
- Is it possible to freeze nuclei prior to Single Cell 3'?
- How can I exclude cells that show enrichment of MT genes from secondary data analysis
- Why do I see more MT enriched cells from Cell Ranger 3 as compared to Cell Ranger 2?
- How can I convert the feature-barcode matrix from Cell Ranger 3.x to a CSV file?
- Can we detect/distinguish different isoforms using single cell 3’ RNA-seq data?
- How can I convert the gene-barcode matrix from Cell Ranger 1.x and 2.x to a CSV file?
- How do I get the read counts for each barcode?