Chromium Controller

• What is the purpose of loading surrogate fluid in the unused wells of the Chromium Chip?
• How do I decontaminate my Chromium Controller?
• What are the specifications of the Chromium Controller?
• How do I clean my Chromium Controller?
• Is the performance of the Chromium affected at high temperatures or humidity?
• What causes a 'Pressure Not At Setpoint' error?

Reagents

• How many freeze thaw cycles are the gel beads stable for?
• Why is it critical to load the wells in the specified order?
• Is there a plate magnetic separator recommended by 10x?
• What is the maximum number of thaw-freeze cycles for the i7 sample index plate?
• Can I mix reagents from different kits?
• What is the shelf life of 10x reagents?

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GEM Generation & Barcoding

• Which thermal cyclers are supported for use with GEMs?

Library Construction

• Can I use a melting curve analysis after the qPCR protocol to assess the quality of my 10x libraries?
• Why do I need to use qPCR to quantify my final library?
• How long can final libraries be stored at -20°C?
• How are SPRIselect volumes calculated?

Sequencing

• Should I increase the number of reads per cell if I increase the number of antibodies?
• Do I need custom sequencing primers for 10x libraries?
• Why are there unequal ratios of the four oligos (or oligo dropout) in my 10x library after sequencing?
• How do you set up a NextSeq run on BaseSpace?
• What oligos are in my sample index?
• Is a sample sheet required for sequencing?

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Software

• [error] Barcode out of bounds (I1:0-14)
• How do I fix the mkfastq error "No bcl2fastq found on path"?
• Is it possible to get gene expression values on a cell by cell basis instead of on a cluster by cluster basis in Loupe Cell Browser?
• Create clusters of a given cell type in different samples in a combined cloupe file
• Is there way to filter the BAM file produced by 10x pipelines with a list of barcodes?
• How do I run a pipeline on multiple libraries?

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