Questions & Answers
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- Why is the distribution of fragments after amplification different between T and B cells?
- Why is the annealing temperature and number of enrichment cycles different for T and B cells?
- How do I design a custom targeted assay for Single Cell V(D)J?
- Why are there two target enrichment steps in the V(D)J workflow?
- Is there a common 5’ UTR captured during V(D)J enrichment?
- Should I adjust the targeted sequencing depth if I did not enrich for T or B cells?
- Can I sequence V(D)J-enriched libraries from T and B cells on the same lane?
- Can I sequence V(D)J and 5' Gene Expression libraries on the same lane?
- Alternative sequencing formats for Single Cell V(D)J libraries
- What Illumina sequencers are supported for Single Cell V(D)J libraries?
- Can I identify differentially expressed genes between cells in two different clonotypes?
- Is the amino acid sequence for the full V(D)J contig available?
- Are the V(D)J and gene expression references interchangeable?
- Will the V(D)J pipeline work without a reference?
- Why is the same TRB chain paired with TRA in clonotype1 and alone in clonotype2?
- Why is the D gene annotation missing in several TCR beta chains?