Question: What if I accidentally use Buffer EB instead of Elution Solution I when eluting from SPRIselect beads in Step 3.2j of the ATAC assay?
Answer: The targeted DNA fragments will be properly eluted from SPRIselect beads if Buffer EB is used instead of Elution Solution I. Elution with Buffer EB in Step 3.2j may result in increased sticking of the SPRIselect beads to the pipet tip. Assay performance will not be affected if care is taken to avoid transferring SPRIselect beads into the new tube strip.
Please note that this guidance applies ONLY to Step 3.2j of the ATAC assay. It does not apply to accidental use of Buffer EB instead of Elution Solution I at any other step or in other assays.
Products: Single Cell ATAC