Question: Which thermal cyclers are compatible with the Visium for fresh-frozen assay?
Answer: The following thermal cyclers have been validated with the Visium for fresh-frozen assay. Validated thermal cyclers have undergone rigorous testing and have generated reproducible, high-quality results when following our 10x Genomics Visium Demonstrated Protocols and User Guides. Please see our Visium Spatial Gene Expression User Guide for general thermal cycler requirements.
Thermal cyclers that are not included in the list above are unsupported as we cannot guarantee assay performance. Untested thermal cyclers may be incompatible with the Thermocycler Adaptor and/or Slide Cassette, which can lead to cassette leakage, broken slides, and the possibility of generating suboptimal data as compared to validated thermal cyclers.
For instance, we have found that the Visium Thermocycler Adaptor doesn’t sit flush with the BioRad T100 thermal cycler block and it generates suboptimal data as compared to supported thermal cyclers. Based upon limited testing with Visium, we observed a decrease in sensitivity (i.e., Genes and UMIs per Spot) and mapping rates when comparing the data generated using a Bio-Rad T100 to a validated thermal cycler.
We would recommend that Visium customers use a validated, supported thermal cycler for performing their Visium experiments for optimal assay performance.
Products: Visium for Fresh-Frozen