Question: Why is my Visium CytAssist Readiness Test failing?
Answer: The Readiness Test assesses instrument hardware functionality and calibrates the imaging system. It should be run during First Time Use (FTU) set-up, after moving the instrument or installing a Firmware update.
In the event of a failed Readiness Test, check the following:
- Confirm that the Shipping Bolt has been removed from the instrument. The Shipping Bolt holds the cameras in position during transportation and if left installed during a run, it may impact optical system calibration.
- If the Shipping Bolt has not yet been removed, unscrew from the back of the instrument. Retain the Shipping Bolt in a marked bag or container in a convenient location in case the instrument requires future transportation.
- Power cycle the instrument.
- Ensure that the instrument, blank slides, and Visium CytAssist Calibration Slide are clean. Dirty alignment windows or slides can negatively impact the Readiness Test. We recommend performing the following steps:
- Wipe the Tissue Slide Stage and the Visium Slide Stage with 5-10% bleach solution followed by 70% ethanol or 70% isopropanol using laboratory wipes. Followed by compressed air to dry and remove debris as needed.
- Clean glass windows with lens paper or laboratory wipes. Use dry polyester swabs (such as Texwipe TX714A Cleanroom Swab, 0.5”L - EW-33677-62 from Cole-Parmer or equivalent) for cleaning window corners. After cleaning, use compressed air to remove fibers and debris.
- Clean the blank slides and the Calibration Slide with lens paper or laboratory wipes followed by compressed air.
- Use a bright light (e.g. flashlight) to inspect the glass windows for any smudges and remove them as described above.
Products: Visium CytAssist