Question: Does the Visium CytAssist Spatial Gene Expression for Fresh Frozen assay require a Tissue Optimization experiment?
Answer: The Visium CytAssist Spatial Gene Expression for Fresh Frozen (FF) assay was developed specifically for FF OCT embedded tissues and was designed with the aim of standardizing permeabilization conditions across tissue types.
- This assay does not require a Tissue Optimization experiment to determine optimal permeabilization time prior to the Visium CytAssist Spatial Gene Expression for FF workflow.
- We expect most human and mouse FF tissues to be compatible with the Visium CytAssist Spatial Gene Expression for FF assay. Customers can consult the Tested Tissue List on the Support Site to see what tissue types have been validated with the assay.
Products: Visium CytAssist for FF