Question: How are the human probes used in the Visium CytAssist Spatial Gene Expression solution similar to and different from those used in the Fixed RNA Profiling solution?
Answer: Below are some similarities and differences between the Human Transcriptome Probes used in the Visium CytAssist Spatial Gene Expression solution compared to the Fixed RNA Profiling solution.
- Both solutions utilize the same strategy of having a small RNA Read 2 (Read 2S) as a handle on the left-hand side (LHS) probes.
- The sequences that Hybridize to Target mRNAs are shared between the two solutions.
- The two solutions use different approaches to capturing the right-hand side (RHS) probes whereby the Visium CytAssist assays use a synthetic poly(A) tail to capture Ligated Probes on the Visium Slide and Fixed RNA Profiling makes use of partial Capture Sequence 1 to capture Ligated Probes by the Gel Bead oligo.
Products: Fixed RNA Profiling Gene Expression, Visium CytAssist for FFPE, Visium CytAssist for FF