Question: How are the human probes used in CytAssisted Visium for FFPE similar to and different from those used in the Fixed RNA Profiling solution?
Answer: Below are some similarities and differences between the Human Transcriptome Probes used in the CytAssisted Visium for FFPE solution compared to the Fixed RNA Profiling solution.
- Both assays use the same strategy of having a small RNA Read 2 (Read 2S) as a handle on the left-hand side (LHS) probes.
- The sequences that Hybridize to Target mRNAs are shared between the two solutions.
- The two assays use different approaches to capturing the right-hand side (RHS) probes whereby CytAssisted Visium for FFPE assay uses a synthetic poly(A) tail to capture Ligated Probes on the Visium Slide and Fixed RNA Profiling makes use of partial Capture Sequence 1 to capture Ligated Probes by the Gel Bead oligo.
Products: Fixed RNA Profiling Gene Expression, Visium CytAssist for FFPE