How are the human probes used in CytAssisted Visium Spatial Gene Expression assays similar to and different from the probes used in the Fixed RNA Profiling solution?

Question: How are the human probes used in CytAssisted Visium Spatial Gene Expression assays similar to and different from those used in the Fixed RNA Profiling solution?

Answer: Below are some similarities and differences between the Human Transcriptome Probes used in the CytAssisted Visium Spatial Gene Expression solutions compared to the Fixed RNA Profiling solution. 

1. Both assays use the same strategy of having a small RNA Read 2 (Read 2S) as a handle on the left-hand side (LHS) probes. 
2. The sequences that Hybridize to Target mRNAs are shared between the two solutions.
3. The two assays use different approaches to capturing the right-hand side (RHS) probes whereby CytAssisted Visium for FFPE assay uses a synthetic poly(A) tail to capture Ligated Probes on the Visium Slide and Fixed RNA Profiling makes use of partial Capture Sequence 1 to capture Ligated Probes by the Gel Bead oligo.

 Products: Fixed RNA Profiling Gene Expression, Visium CytAssist for FFPE, Visium CytAssist for FF