Question: Can Visium CytAssist Spatial Gene Expression libraries be pooled with other 10x libraries?
Answer: Visium CytAssist Spatial Gene Expression libraries can be combined with other 10x dual-index libraries.
- If combining with Single Cell or Visium for Fresh-frozen libraries, Read 2 should be increased to 90 cycles. When pooling in this fashion, we recommend limiting Visium CytAssist Spatial Gene Expression libraries to <40% of the total sequencing pool to maximize quality.
- Libraries created with the same sample index should not be pooled together.
Products: Visium CytAssist for FFPE, Visium CytAssist for FF, Visium CytAssist for FxF