Question: What are the minimum recommended sequencing specifications for Visium CytAssist Spatial Gene Expression libraries?
Answer: We recommend sequencing at least 25k read pairs per tissue-covered spot. Visium CytAssist Spatial Gene Expression libraries can be sequenced with 100 cycle kits and above.
Visium CytAssist Spatial Gene Expression libraries should be run in paired-end, dual index configuration. We recommend the following cycling conditions:
Read 1 - 28 cycles
i5 index - 10 cycles
i7 index - 10 cycles
Read 2S - 50 cycles
If combined with dual indexed Single Cell or Visium for Fresh-frozen libraries, Read 2 should be increased to 90 cycles.
Products: Visium CytAssist for FFPE, Visium CytAssist for FF