Question: What thermal cyclers are validated with the Visium CytAssist for FFPE solution?
Answer: We have validated the following thermal cyclers:
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- BioRad C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module
- Eppendorf MasterCycler Pro
- Eppendorf MasterCycler X50s
- Thermo Fisher Scientific Veriti 96-Well Thermal Cycler
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Below are the minimum thermal cycler specifications if reactions are performed on a tube:
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- Uniform heating of 100 µl volumes
- Temperature-controlled lid
- 96 deep-well block or 0.2 ml block configuration
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Below are the minimum thermal cycler specifications for compatibility if reactions are performed on a slide. The thermal cycler must be able to accommodate the Low Profile Thermocycler Adapter:
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- Well depth: 4.5 mm
- Distance between block and heated lid: 12 mm
- Reaction block dimensions: 95.5 x 73 mm
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Products: Visium CytAssist for FFPE