Question:How many antibodies have we simultaneously tested following the IF Staining Demonstrated Protocol?
Answer: In house we have tested simultaneously staining mouse and human tissues with up to 3 antibodies plus DAPI. The analysis pipeline can process up to 6 channels.
It is important that customers perform antibody concentration optimization prior to processing their samples of interest to achieve desired staining. The steps for doing optimization are outlined in the IF Staining & Imaging Demonstrated Protocol.
Products: Visium CytAssist for FFPE