Question: How do I determine if my tissue block or archived section will be compatible with the Visium for FFPE assay with the CytAssist?
Answer: We are recommending that customers perform an RNA Quality Assessment of their tissue block of interest or scraping a representative section from an archived slide before the Visium CytAssist for FFPE Gene Expression assay.
Based on in-house experiments, we recommend using tissue with DV200% ≥30%. Using low DV200-scored blocks will still yield data but likely of lower sensitivity (i.e., lower UMIs and Genes per Spot).
Details on how to perform RNA Quality Assessment are in our Tissue Preparation Guide.
Products: Visium CytAssist for FFPE