Question: Can I use pre-stained slides with CytAssist?
Answer: CytAssist is compatible with H&E or IF (IF & DAPI) stained FFPE tissues pre-sectioned on standard histology slides. The assay is also compatible with archived slides that have been hard coverslipped.
Assay performance will depend on the quality of the RNA in the tissue, which in turn will depend on several factors, including the fixation and embedding method, storage conditions, and age of the slide. We recommend performing an RNA quality assessment after hard coverslip removal from archived slides. Additional details regarding processing archived slides can be found in the Visium CytAssist Spatial Gene Expression for FFPE – Tissue Preparation Guide.
Products: Visium CytAssist for FFPE