Question: How does the CytAssist workflow compare to the Visium for FFPE v1 workflow?
Answer: CytAssist workflow begins with the use of tissues on supported standard glass slides within the allowable area. Upstream workflow steps are performed on plain glass slides. CytAssisted probe transfer to Visium Slides with 2 capture areas.
Two images are captured, one using a microscope after staining and one by the instrument, capturing the tissue with the Visium Fiducial Frames. Inside the instrument, CytAssist controls the process of tissue permeabilization and allows for the transfer of Ligated Probes to the Visium Slide.
Probe Extension, Library Construction, and sequencing steps are performed similarly to the Visium for FFPE workflow.
Products: Visium CytAssist for FFPE