Question: Is it possible to aggregate if we obtain nuclei and cellular data from the same sample?
Answer: Yes, it is possible to run
cellranger aggr on nuclei and cellular data for the same sample. If running
cellranger count or
--include-introns and if using the same reference on both these data types, there are no potential batch effects. It is also the recommended method to run
cellranger aggr. In a few edge cases, the results are dependent on the cell type and tissue complexity, and using third-party tools like Seurat may be considered as these tools simply merge the raw feature barcode matrices and normalizes them. Please note that it is not possible to aggregate cellular datasets run without intron-mode with nuclei datasets.
How does the Gene Expression data compare between whole cells and nuclei?
Products: Single Cell Gene Expression