Question: How do I know when my tissue is fully dissociated using the Chromium Nuclei Isolation Kit?
Answer: Continue dissociating the tissue until the lysate appears homogeneous and no large pieces of tissue remain. After dissociation, if the tissue is unable to pipette mix before incubation in lysis buffer on ice, then continue to dissociate the tissue with the pestle until able to pipette mix.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Single Cell Multiome ATAC + GEX, Fixed RNA Profiling