Question: What are the recommendations when using a high RNase tissue when isolating nuclei using the Chromium Nuclei Isolation Kit?
Answer: The following recommendations are made when using high RNAse tissue:
- The RNase inhibitor volume used in the Nuclei Isolation kit was optimized to work with a wide range of samples, including some high RNase tissues. We suggest following the protocol as is before attempting to do further optimization.
- Collection interval, freezing, and storage conditions can impact tissue quality, especially for high RNase tissues. It is recommended to freeze tissues as soon as possible post collection to reduce the impact of RNases. Avoid freezing and thawing cycles and store in LN to minimize RNase activity.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Single Cell Multiome ATAC + GEX, Fixed RNA Profiling