Question: What are the recommendations when using a limited sample when isolating nuclei using the Chromium Nuclei Isolation Kit?
Answer: If using a limited sample and nuclei recovery is expected to be low, a single wash may be used in an attempt to improve recovery. In addition to a single wash, increasing centrifugation time during wash steps can improve nuclei yield. The use of swing-bucket rotors, which pellet nuclei to the bottom of tubes rather than the side, may also improve final nuclei recovery
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