Question: Do I need to run a lysis timeline using the Chromium Nuclei Isolation kit?
Answer: The Chromium Nuclei Isolation Kit was optimized using many human and mouse samples and is expected to be compatible with most mammalian tissues without lysis time optimization. We suggest following the protocol directly when working with a new sample type.
For optimization of non-tested tissue types, please see: How can I optimize my nuclei suspension if I am using an untested tissue type in the Chromium Nuclei Isolation kit?
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Single Cell Multiome ATAC + GEX, Fixed RNA Profiling