Question: What are the best practices for tissue storage and handling before using the Chromium Nuclei Isolation Kit?
Answer: Tissues should be rinsed with 1x PBS to remove blood and other debris, patted dry and frozen as quickly as possible after harvest. To flash freeze (also known as snap freeze), place the tissue in a tube for long term storage (cryotube or Eppendorf tube with parafilm wrapped around the top) and either submerge the tube in liquid nitrogen or a liquid-nitrogen cooled bath (e.g., isopentane) or place the tube deep in a bucket of dry ice. Wait at least 2-3 minutes for the tissue to completely freeze, and transfer the tube containing the tissue to vapor-phase liquid nitrogen for long-term storage. For shorter-term storage, the tissue may be stored at -80C.
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