Question: Do I need to vortex my nuclei to dissociate aggregates during the Chromium Nuclei Isolation workflow?
Answer: During the Chromium Nuclei Isolation workflow, two steps in the protocol require vortexing nuclei. The vortex mixing during these designated steps is critical for the dissociation of nuclei aggregates and accurate nuclei counting. The isolation protocol can isolate nuclei in one hour, ensuring the nuclei are robust and able to undergo brief vortexing to prevent nuclei aggregation.
Pipette mixing during this protocol can result in variability from user to user and lead to insufficient mixing and nuclei aggregation.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Single Cell Multiome ATAC + GEX, Fixed RNA Profiling