Question: Do I need to vortex my nuclei to dissociate aggregates during the Chromium Nuclei Isolation workflow?
Answer: During the Chromium Nuclei Isolation workflow, two steps in the protocol require vortexing nuclei. The vortex mixing during these designated steps is critical for the dissociation of nuclei aggregates and accurate nuclei counting. The isolation protocol can isolate nuclei in one hour, ensuring the nuclei are robust and able to undergo brief vortexing to prevent nuclei aggregation.
For samples with low nuclei yields, the final nuclei pellet can be resuspended in 25ul Resuspension Buffer and mixed ~10 times with a narrow bore pipette tip instead of vortexing to increase the concentration of the final nuclei suspension.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Single Cell Multiome ATAC + GEX, Fixed RNA Profiling