Question: What is the tissue size range that I can use with the Chromium Nuclei Isolation Kit?
Answer: Supported tissue sizes start from 3-50mg per column. Larger tissues may be run by splitting into smaller pieces and running separate reactions. It may be possible to run tissues that are <3mg with the expectation that this may yield lower nuclei recovery.
Shown above is a visual representation of the appearance of different masses of mouse kidney and heart tissue.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Single Cell Multiome ATAC + GEX, Fixed RNA Profiling