Question: Why are cell cultures and cell suspensions not compatible with the Chromium Nuclei Isolation Kit?
Answer: Cell cultures and cell suspensions are not compatible with the Chromium Nuclei Isolation Kit because:
- The Chromium Nuclei Isolation kit was optimized for nuclei isolation from frozen tissue samples.
- Due to fundamental differences between these sample types, tissues and cell suspensions require different lysis conditions to isolate nuclei.
- Isolating nuclei from cell suspensions compared to tissue samples will result in different levels of debris, with tissues necessitating more cleanup than cell suspensions.
- Most nuclei isolation protocols for cell suspensions are straightforward and do not contain the additional cleanup steps included in the Chromium Nuclei Isolation Kit workflow.
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