Question: How does the Chromium Nuclei Isolation Kit work?
Answer: The Chromium Nuclei Isolation Kit utilizes the following steps:
- Tissues are dissociated using a pestle and an optimized lysis solution
- Nuclei are isolated from tissue using a Nuclei Isolation Column
- Nuclei suspensions are cleaned with a Debris Removal Solution and two subsequent washes
- Nuclei are resuspended, counted, and ready to use with compatible 10x Genomics single cell assays
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Single Cell Multiome ATAC + GEX, Fixed RNA Profiling