Question: Is there any additional guidance for fixing Whole Blood using the Fixed RNA Profiling assay?
Answer: We currently do not have any recommendations or guidelines for fixing whole blood using the Fixed RNA Profiling (i.e., Single Cell Gene Expression Flex) assay. We hope to develop a demonstrated protocol for fixing whole blood followed by RBC depletion. However, this is still under development, and we do not have an estimated time as to when this will become available. Leukocytes, bone marrow mononuclear cells (BMMCs), and peripheral blood mononuclear cells (PBMCs) can be isolated using CG000392. The isolation and fixation of PBMC have been extensively tested.
Products: Fixed RNA Profiling Gene Expression, Single Cell Gene Expression Flex