Question: Is there any additional guidance for fixing Whole Blood using the Fixed RNA Profiling assay?
Answer: If interested in fixing whole blood for use with the Fixed RNA Profiling Assay (i.e., Single Cell Gene Expression Flex assay), users should refer to the Blood Fixation and Cell Isolation for Chromium Fixed RNA Profiling Demonstrated Protocol (CG000721).
This protocol outlines fixation of blood and isolation of peripheral blood mononuclear cells (PBMCs) and leukocytes from the fixed blood for use with the Chromium Fixed RNA Profiling workflow. Storage recommendations for the PBMCs isolated from fixed blood and post-storage processing conditions are also provided. An overview of data derived from fixed PBMCs and leukocytes used with the Chromium Fixed Profiling workflow is also shown.
Products: Fixed RNA Profiling Gene Expression, Single Cell Gene Expression Flex