Question: Are neutrophils and other granulocytes compatible with the Fixed RNA Profiling Assay?
Answer: We have performed very limited testing in-house of neutrophils or other granulocytes in the Fixed RNA Profiling Assay. Fixation of these samples should limit the degradation after collection that is often observed due to high levels of RNases and other inhibitory compounds. Similar considerations exist for sample preparation before fixation, as with other assays.
Neutrophils should be fixed immediately after collection to limit degradation after collection. For additional sample preparation guidance, please see: Can I process neutrophils (or other granulocytes) using 10x Single Cell applications?
Products: Fixed RNA Profiling Gene Expression