Question: Can alternative buffers or fixation protocols be used for sample fixation?
Answer: We have not tested the use of alternative buffers or fixation protocols with the Fixed RNA Profiling assay, and thus cannot provide any specific recommendations.
Other fixation buffers containing formaldehyde at a final concentration of 4% may be compatible with the Fixed RNA Profiling assay but they are untested and unsupported. We advise performing a small pilot study before committing to large-scale experiments.
If the formaldehyde validated in the 10x Demonstrated Protocol Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling or Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling is not available in your region, any Molecular Biology Grade stock solution of 37% formaldehyde that is ~10% methanol stabilized (with little to no precipitant) may be substituted.
Products: Fixed RNA Profiling Gene Expression