Question: What sequencing parameters should be used for Fixed RNA Profiling gene expression libraries?
Answer: Fixed RNA Profiling gene expression libraries should be run paired-end, dual index configuration on the recommended Illumina sequencers. We recommend the following sequencing cycling conditions:
Read 1 - 28 cycles
i7 index - 10 cycles
I5 index - 10 cycles
Read 2 - 90 cycles
(Minimum required Read 2 length is 76 bp in Multiplexed libraries and 50 bp in Singleplex libraries).
For additional information, please review Sequencing Requirements for Fixed RNA Profiling.
Products: Fixed RNA Profiling Gene Expression, Single Cell Gene Expression Flex