Question: What dual index plate is compatible with the Chromium Fixed RNA workflow?
Answer: The Dual Index Kit Plate TS Set A, 96 rxns (PN-1000251) is the Sample Index Plate required for use with the Chromium Fixed RNA Profiling (i.e., Single Cell Gene Expression Flex) workflow.
Each well of the Dual Index Plate TS Set A (PN-1000251) contains a mix of one unique i7 (Index 1) and one unique i5 (Index 2) sample index.
Each sample index well in the 96-well plate is a unique set, and therefore the sample indexes can be used in any combination. Thus, if you choose a unique well from the 96-well plate for each sample that will be multiplexed, the samples can be run on a single flow cell lane.
Fixed RNA Profiling Gene Expression libraries are made using the following sample index plate:
- Fixed RNA Profiling Cell Surface Protein libraries (TotalSeq™️-B) are made using the following sample index plate:
- Fixed RNA Profiling Cell Surface Protein libraries (TotalSeq™️-C; Barcode Oligo Capture) are made using the following sample index plate:
Note: The 10x Sample Index sequences are not needed when setting up the sample sheet, but the 10x Sample Index name (i.e., SI-TS-A1) is needed for demultiplexing sequencing runs when running more than one sample per lane.
Products: Fixed RNA Profiling Gene Expression, Single Cell Gene Expression Flex