Question: What are the highest spin speeds and spin times recommended for nuclei isolation for Single Cell ATAC or Single Cell Multiome assays?
Answer: The highest spin speed recommended for nuclei isolation is 500xg. The spin times we optimized in our demonstrated protocols are 5 min per wash at 4°C. If nuclei yield is an issue, the spin time may be increased. However, we do not recommend increasing the spin speed as it may adversely affect nuclei sample quality.
Products: Single Cell ATAC, Single Cell Multiome ATAC+GEX