Question: Is the Slide Reset Demonstrated Protocol (CG000332) compatible with the Visium Spatial Gene Expression for FFPE assay?
Answer: The Slide Reset Demonstrated Protocol (CG000332) is NOT compatible with FFPE tissues and should only be used with fresh frozen tissue. We have tried to adapt this protocol to work with FFPE tissues but have found that it is, unfortunately, incompatible.
We would recommend practicing sectioning and placement prior to proceeding with the Gene Expression experiment. Please see the following resources for guidance:
- How do I practice sectioning and tissue placement for Visium for FFPE?
- How can I easily visualize the Fiducial Frame on the Visium Slide during tissue section placement?
- How-To Video on Practicing Section Placement of Plain Glass Slide
Products: Visium for FFPE