Question: How much amplified DNA from Feature Barcode can be collected during the automated 5' V2 and automated 5' cDNA workflows?
Answer: For both the Automated 5' v2 GEX Kit and 5' cDNA Kit v2, the enablement of collecting the Feature Barcode product will instruct the user to load the appropriate number of tube strips for removal during certain points in the assay.
For Chromium Next GEM Automated Single Cell 5' v2 GEX Kit
- Enabling the feature barcode reaction will allow for obtaining a separate aliquot of 130 uL of the amplified Feature Barcode DNA during the cDNA QC step into tube strip 2.
- This will be available during the cDNA QC portion of the assay, and the user interface will prompt the user to remove the Feature Barcode DNA product for storage.
For Chromium Next GEM Automated Single Cell 5' cDNA Kit v2
- Enabling the feature barcode reaction will allow for obtaining a separate aliquot of 130 uL of the amplified Feature Barcode DNA during the completion of the run in tube strip 2 when the user collects the cDNA in tube strip 1.
- The Amplified Feature Barcode DNA product will be available for collection during the end of the cDNA-only workflow.
Products: Chromium Connect