Question: Is 5' CRISPR compatible with Cas12/Cas13 CRISPR libraries?
Answer: The 5’ CRISPR Kit is specifically designed for functionality with CRISPR-Cas9 guide libraries. Other Cas enzyme libraries may be compatible with the 5’ assay in conjunction with a custom spike-in primer (see ‘My guide is not compatible with the 5' CRISPR, how do I design a custom primer?’); however, this relies on the protospacer being located on the 5’ end of the sgRNA, as in the image below.
For Cas systems where the protospacer is located at the 3’ end of the sgRNA (such as Cas12a or Cas13a), it is not possible to design a custom primer, as this would be downstream of the protospacer sequence and therefore would not capture it during reverse transcription.
Product: Single Cell Immune Profiling