Question: What is the expected size of my 5’ CRISPR library?
Answer: The 5’ CRISPR Screening final library will vary in size depending on the guide design. We expect to observe a single, narrow peak at ~270bp in CRISPR Screening libraries generated from guides using our 5’ Immune Profiling or 5’ HT Immune Profiling with CRISPR Screening protocols (as shown below).
The library size can vary slightly depending on the guide of interest (~270-300 bp peak).
Product: Single Cell Immune Profiling