Question: What comes with my 5’ CRISPR kit?
Answer: 5’ CRISPR kit (PN-1000451) contains four tubes.
- CRISPR Poly-dT RT primer mix (PN-2000593) is necessary for sgRNA amplification during reverse transcription.
- Feature SI Primer 4 (PN-2000592) is necessary for sgRNA final library amplification along with the supplied amplification mix (PN-2000047) and the Dual Index Kit TT Set A (PN-1000215).
This kit is sufficient for generating 16 5’ CRISPR libraries with either the 5’ v2 standard or 5’ HT v2 chemistry.
Product: Single Cell Immune Profiling