I have resequenced the Gene Expression library multiple times and all my attempts to run it on 10x Genomics Cloud Analysis failed. How to analyze this dataset on Cloud?
When there is a mixture of read lengths in a dataset, the following is an example error message observed from the analysis logs:
We detected an unsupported chemistry combination (SC5P-R2, SC5P-PE). To process this combination of data you will need to specify SC5P-R2 via the --chemistry argument.
In such cases of mixed read lengths, autodetect chemistry will not work and the user is required to manually specify chemistry by selecting SC5P-R2 option and then proceed to run analysis. The dropdown menu for selecting chemistry can be found in the Advanced Settings section.
10x Genomics Cloud Analysis is only available in the United States and Canada.
Products: Single Cell Gene Expression
Related Articles: How to specify chemistry in 10x Genomics Cloud Analysis?