I have resequenced the Gene Expression library multiple times and all my attempts to run it on 10x Genomics Cloud Portal failed. How to analyze this dataset on Cloud?
When there is a mixture of read lengths in a dataset, the following is an example error message observed from the analysis logs:
We detected an unsupported chemistry combination (SC5P-R2, SC5P-PE). To process this combination of data you will need to specify SC5P-R2 via the --chemistry argument.
In such cases of mixed read lengths, autodetect chemistry will not work and the user is required to manually specify chemistry by selecting the
--chemistry parameter and then proceed to submit the Cloud Cell Ranger jobs.
Products: Single Cell Gene Expression