Question: Are tissue microarrays (TMAs) supported with Visium for FFPE?
Answer: We currently support one tissue section per capture area on the Visium Slide. Therefore, TMA’s are not currently officially supported for our Visium for FFPE solution. We leave the decision to proceed with such specimens at the customer’s discretion. However, such samples are unsupported and performance cannot be guaranteed.
Below are some challenges when working with TMAs:
- Section floating and getting wrinkles out may be more technically challenging given that the optimal float times/temperatures may be variable
- The detachment of one core could negatively impact others in the same capture area
- Data analysis could be challenging depending on the number of cores (you'd have to select the entire capture area or individually manually align them)
- Lateral diffusion can occur at the tissue edges and this theoretically could lead to contamination if the cores are very close
Product: Visium for FFPE